FM Minimal medium w/o Glutamic acid and w/o Lysine Size:1kg

FM Minimal medium w/o Glutamic acid and w/o Lysine Size:1kgThis media is based on well defined formulations of mineral salts, vitamins and amino acids. Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH. FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5. SIH medium is a newly developed modification of FM with regards to

MOPS is frequently used as a buffering agent in biology and biochemistry

H2O) Clear and colourless solution pH (5%

186 Agar 15 Final pH 7

15 (NH4)6Mo7O24

inhibiting protein synthesis and generating errors in the transcription of the genetic code

84 ZnSO4 2

Recombinant E

Tetracycline is a bacteriostatic antibioticum with activity against gram-positive and gram-negative bacteria

2 at 25°C Suspend 15

Friehs and Flaschel (2004)

5 Glucose 5 KH2PO4 2

Yeast extract is a rich source of amino acids