Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Thiamine Size:500g 2 Glucose 2 KH2PO4 1

2 Glucose 2 KH2PO4 1

Ampicillin is an Inhibitor of bacterial cell wall synthesis and inhibits crosslinking of peptidoglycan by binding and inactivating of transpeptidases

2 at 25°C Suspend 60 gram powdered medium in 1 litre distilled water

Tryptone 10 Yeast extract 5 NaCl 5 MgSO4 anhydrous 0

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Thiamine Size:500g 2 Glucose 2 KH2PO4 1Thiamine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of thiamine or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room