Translucent K+ free medium”, YNB w/o Amino acids and without Ammonium sulphate and w/o Potassium

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Description

Translucent K+ free medium”, YNB w/o Amino acids and without Ammonium sulphate and w/o PotassiumIn Translucent K free medium ammonium phosphate (0. 92 g l) substitutes for potassium phosphate to reduce the amount of potassium to a minimum. Suspend 1. 75 gram powdered medium in 1 litre distilled water Store dry at room temperature

Both Folic acid and Riboflavin are omitted from the medium to make it suitable for fluorescence measurements with yeast cultures

Chemical formula C6H13NO2 Solubility (20° C) 24 g/l Mol

Manganese sulphate

49 Suspend 24

permitting incubation without melting while its transparency allows easy observation of colonies

The medium can be supplemented with specific amino acids or other required nutrients allowing for selection of specific auxotrophs

The antibiotic inhibits peptide chain elongation by preventing elongation-factor EF-2 dependent translocation

As a result yeasts are not only limited in Phosphate

Chemical formula C6H12O6 Solubility (20° C) 250 g/l Mol

0 L-Threonine 47

C8H18N2O4S=238

3% Water <= 6