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Description

AX MediumAX Medium is used to induce and to cultivate an axenic culture of Dictyostelium discoideum from bacterially grown cells. Component g l Peptone 14. 3 Yeast Extract 7. 15 Glucose 18 KH2PO4 0. 49 Na2HPO4. anhydrous 0. 49 Suspend 40. 4 gram powdered medium in 1 litre distilled water. Store dry at room temperature

The latter containing trypsin

These Amino acids form a basis for many "Drop-Out" mixtures to select for auxotrophic requirements and transformants

4 (100 g/l) Assay ≥99%

phenylalanine and glycine

Current Protocols

Nitrogen is provided by ammonium chloride final osmolarity of the medium is maintained by NaCl

The antibiotic inhibits peptide chain elongation by preventing elongation-factor EF-2 dependent translocation

K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium

LBM-Broth Low Salt

5 X sterile stock solution

Amino Acids Arg 700 Asp 300 Cys 200 GluA 500 Gly 900 His 300 Ile 600 Leu 900 Lys 900 Met 300 Phe 500 Pro 800 Thr 500 Trp 200 Val 700 Vitamins Biotin 0

Non-toxic to plant cells

Exchange/Return Notes