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M9 Minimal Salts Base, 5xM9 Minimal Salts Base, 5x is used in preparing M9 Minimal medium used for the cultivation and maintenance of E. coli in molecular biology. M9 is a chemical defined minimal medium supporting the growth of "wild type" strains of E. coli. The medium can be supplemented with specific amino acids or other required nutrients allowing for selection of specific auxotrophs. Glucose may be added as a source of carbohydrate. Nitrogen is provided by ammonium

In LBM media Mg2 is added to enhance absorption of phage lambda to the cells

The antibiotic interferes with the translation step of polypeptide synthesis of prokaryotes and eukaryotes

M9 is a chemical defined minimal medium supporting the growth of "wild-type" strains of E

Suspend 250

3g) in one litre is 10X

2 Na2HPO4 0

9 gram powdered medium in 1 litre distilled water

Water) Clear APHP Color

Suspend 36

Chemical formula C6H12O6 Solubility (20° C) 250 g/l Mol

Respiratory deficient and pet mutants will not grow on YPEG

HL5 supplemented with vitamins and micro-elements as present in FM and SIH Media