Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Sodium

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Description

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without SodiumSodium chloride, NaCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Sodium or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 8 gram powdered medium in 1 litre distilled water Store dry at room

All nutritional requirements of E

925 – 930

3% Appearance White Crystalline Powder Store dry at room temperature

Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption

The concentration of NaCl in LB Lennox is half the concentration of the LB Miller Broth formulation and ten times the concentration of LB Luria Broth formulation

Adenine 50 L-Histidine HCl 50 L-Leucine 50 Uracil 50

Final concentration is 0

Adenine 50 L-Histidine HCl 50 L-Leucine 50 L-Lysine HCl 50 Uracil 50

Luria Broth

Amino Acids Arg 700 Asp 300 Asp A 150 Cys 300 GluA 545 Gly 900 His 300 Ile 600 Leu 900 Lys 1250 Met 350 Phe 550 Pro 800 Thr 500 Trp 350 Val 700 Vitamins Biotin 0

VL6 is a complex medium for cultivation of Dictyostelium discoideum based on Vegetable Peptone

Add 2 - 4x for auxotrophs or slower growing strains

Exchange/Return Notes